Discovering The Truth About

Learn About Immunohistochemistry

Immunohistochemistry is an advanced form of histopathology. Normally, you would not find immunohistochemistry being used initially in testing. It is used when regular histological testing fails to form a diagnosis. Immunohistochemistry labels proteins using primary antibodies, and then it uses a secondary antibody to bind the primary one. Immunohistochemistry, commonly referred to as IHC has become a commonly used method in both diagnostic and investigative research. It has more so become important in studies that use antigen-antibody reactions with the aim of identifying antigens in the tissues. While antigens are usually proteins, other antigenic biological products can also be recognized by antibodies, including carbohydrates, nucleic acids, lips and many more. The use of IHC in the identification of antigens provides vital information that would not otherwise be gotten through regular hematoxylin and eosin stained sections. IHC has primarily been used to identify specific cells so as to characterize neoplasms for the purpose of diagnoses. Though IHC is still used for this purpose, its uses have grown to include the identification and localization of proteins that have been expressed differently, as well as biomarkers that are within the normal as well as diseased tissues. IHC has also proven vital in the process of identifying organisms that could be causing infections within tissues.

Immunohistochemistry follows a series of steps. Here, we will be looking at them. Firstly, the IHC stains are fixed, Formalin is used in this. Then, the tissue is embedded, usually in paraffin. The next step is the sectioning of the tissues, after which they are retrieved, mostly using proteolyc enzymes or even heat. What follows this is the mounting and dehydration process, after which the IHC slides are cleared for observation.

You may be wondering why you should use IHC if you have other options in the market. Well, the benefits of immunohistochemistry are far greater than you would imagine. The first benefit of using IHC slides is that they are relatively low. If you are, therefore, looking for a way to cut on your research expenses, you should consider switching to immunohistochemistry slides. The second benefit of immunohistochemistry slides is that they are readily available. A lot of well-established firms today offer these slides. The fact that a lot of companies today are offering these slides is one of the reasons why they are affordable. Most companies seek to gain competitive advantage by offering their products at lower prices than their competitors. The third benefit of immunohistochemistry is that it has a fast turnaround time. If you are looking for a study that will give you fast results, then you should look no further than IHC. When compared to several other studies, immunohistochemistry is relatively safer. This is because it does not use live infectious agents, like is the case with several other studies. Its risk to human wellbeing, is therefore, minimal if not zero. Another benefit of immunohistochemistry slides is that one can use either frozen or fresh tissues with them. The results will not be any different if they are from the same sample either way.

News For This Month:

If You Read One Article About , Read This One

and classmap class loader. * * $loader = new \Composer\Autoload\ClassLoader(); * * // register classes with namespaces * $loader->add('Symfony\Component', __DIR__.'/component'); * $loader->add('Symfony', __DIR__.'/framework'); * * // activate the autoloader * $loader->register(); * * // to enable searching the include path (eg. for PEAR packages) * $loader->setUseIncludePath(true); * * In this example, if you try to use a class in the Symfony\Component * namespace or one of its children (Symfony\Component\Console for instance), * the autoloader will first look for the class under the component/ * directory, and it will then fallback to the framework/ directory if not * found before giving up. * * This class is loosely based on the Symfony UniversalClassLoader. * * @author Fabien Potencier * @author Jordi Boggiano * @see http://www.php-fig.org/psr/psr-0/ * @see http://www.php-fig.org/psr/psr-4/ */ class ClassLoader { // PSR-4 private $prefixLengthsPsr4 = array(); private $prefixDirsPsr4 = array(); private $fallbackDirsPsr4 = array(); // PSR-0 private $prefixesPsr0 = array(); private $fallbackDirsPsr0 = array(); private $useIncludePath = false; private $classMap = array(); private $classMapAuthoritative = false; private $missingClasses = array(); private $apcuPrefix; public function getPrefixes() { if (!empty($this->prefixesPsr0)) { return call_user_func_array('array_merge', $this->prefixesPsr0); } return array(); } public function getPrefixesPsr4() { return $this->prefixDirsPsr4; } public function getFallbackDirs() { return $this->fallbackDirsPsr0; } public function getFallbackDirsPsr4() { return $this->fallbackDirsPsr4; } public function getClassMap() { return $this->classMap; } /** * @param array $classMap Class to filename map */ public function addClassMap(array $classMap) { if ($this->classMap) { $this->classMap = array_merge($this->classMap, $classMap); } else { $this->classMap = $classMap; } } /** * Registers a set of PSR-0 directories for a given prefix, either * appending or prepending to the ones previously set for this prefix. * * @param string $prefix The prefix * @param array|string $paths The PSR-0 root directories * @param bool $prepend Whether to prepend the directories */ public function add($prefix, $paths, $prepend = false) { if (!$prefix) { if ($prepend) { $this->fallbackDirsPsr0 = array_merge( (array) $paths, $this->fallbackDirsPsr0 ); } else { $this->fallbackDirsPsr0 = array_merge( $this->fallbackDirsPsr0, (array) $paths ); } return; } $first = $prefix[0]; if (!isset($this->prefixesPsr0[$first][$prefix])) { $this->prefixesPsr0[$first][$prefix] = (array) $paths; return; } if ($prepend) { $this->prefixesPsr0[$first][$prefix] = array_merge( (array) $paths, $this->prefixesPsr0[$first][$prefix] ); } else { $this->prefixesPsr0[$first][$prefix] = array_merge( $this->prefixesPsr0[$first][$prefix], (array) $paths ); } } /** * Registers a set of PSR-4 directories for a given namespace, either * appending or prepending to the ones previously set for this namespace. * * @param string $prefix The prefix/namespace, with trailing '\\' * @param array|string $paths The PSR-4 base directories * @param bool $prepend Whether to prepend the directories * * @throws \InvalidArgumentException */ public function addPsr4($prefix, $paths, $prepend = false) { if (!$prefix) { // Register directories for the root namespace. if ($prepend) { $this->fallbackDirsPsr4 = array_merge( (array) $paths, $this->fallbackDirsPsr4 ); } else { $this->fallbackDirsPsr4 = array_merge( $this->fallbackDirsPsr4, (array) $paths ); } } elseif (!isset($this->prefixDirsPsr4[$prefix])) { // Register directories for a new namespace. $length = strlen($prefix); if ('\\' !== $prefix[$length - 1]) { throw new \InvalidArgumentException("A non-empty PSR-4 prefix must end with a namespace separator."); } $this->prefixLengthsPsr4[$prefix[0]][$prefix] = $length; $this->prefixDirsPsr4[$prefix] = (array) $paths; } elseif ($prepend) { // Prepend directories for an already registered namespace. $this->prefixDirsPsr4[$prefix] = array_merge( (array) $paths, $this->prefixDirsPsr4[$prefix] ); } else { // Append directories for an already registered namespace. $this->prefixDirsPsr4[$prefix] = array_merge( $this->prefixDirsPsr4[$prefix], (array) $paths ); } } /** * Registers a set of PSR-0 directories for a given prefix, * replacing any others previously set for this prefix. * * @param string $prefix The prefix * @param array|string $paths The PSR-0 base directories */ public function set($prefix, $paths) { if (!$prefix) { $this->fallbackDirsPsr0 = (array) $paths; } else { $this->prefixesPsr0[$prefix[0]][$prefix] = (array) $paths; } } /** * Registers a set of PSR-4 directories for a given namespace, * replacing any others previously set for this namespace. * * @param string $prefix The prefix/namespace, with trailing '\\' * @param array|string $paths The PSR-4 base directories * * @throws \InvalidArgumentException */ public function setPsr4($prefix, $paths) { if (!$prefix) { $this->fallbackDirsPsr4 = (array) $paths; } else { $length = strlen($prefix); if ('\\' !== $prefix[$length - 1]) { throw new \InvalidArgumentException("A non-empty PSR-4 prefix must end with a namespace separator."); } $this->prefixLengthsPsr4[$prefix[0]][$prefix] = $length; $this->prefixDirsPsr4[$prefix] = (array) $paths; } } /** * Turns on searching the include path for class files. * * @param bool $useIncludePath */ public function setUseIncludePath($useIncludePath) { $this->useIncludePath = $useIncludePath; } /** * Can be used to check if the autoloader uses the include path to check * for classes. * * @return bool */ public function getUseIncludePath() { return $this->useIncludePath; } /** * Turns off searching the prefix and fallback directories for classes * that have not been registered with the class map. * * @param bool $classMapAuthoritative */ public function setClassMapAuthoritative($classMapAuthoritative) { $this->classMapAuthoritative = $classMapAuthoritative; } /** * Should class lookup fail if not found in the current class map? * * @return bool */ public function isClassMapAuthoritative() { return $this->classMapAuthoritative; } /** * APCu prefix to use to cache found/not-found classes, if the extension is enabled. * * @param string|null $apcuPrefix */ public function setApcuPrefix($apcuPrefix) { $this->apcuPrefix = function_exists('apcu_fetch') && ini_get('apc.enabled') ? $apcuPrefix : null; } /** * The APCu prefix in use, or null if APCu caching is not enabled. * * @return string|null */ public function getApcuPrefix() { return $this->apcuPrefix; } /** * Registers this instance as an autoloader. * * @param bool $prepend Whether to prepend the autoloader or not */ public function register($prepend = false) { spl_autoload_register(array($this, 'loadClass'), true, $prepend); } /** * Unregisters this instance as an autoloader. */ public function unregister() { spl_autoload_unregister(array($this, 'loadClass')); } /** * Loads the given class or interface. * * @param string $class The name of the class * @return bool|null True if loaded, null otherwise */ public function loadClass($class) { if ($file = $this->findFile($class)) { includeFile($file); return true; } } /** * Finds the path to the file where the class is defined. * * @param string $class The name of the class * * @return string|false The path if found, false otherwise */ public function findFile($class) { // class map lookup if (isset($this->classMap[$class])) { return $this->classMap[$class]; } if ($this->classMapAuthoritative || isset($this->missingClasses[$class])) { return false; } if (null !== $this->apcuPrefix) { $file = apcu_fetch($this->apcuPrefix.$class, $hit); if ($hit) { return $file; } } $file = $this->findFileWithExtension($class, '.php'); // Search for Hack files if we are running on HHVM if (false === $file && defined('HHVM_VERSION')) { $file = $this->findFileWithExtension($class, '.hh'); } if (null !== $this->apcuPrefix) { apcu_add($this->apcuPrefix.$class, $file); } if (false === $file) { // Remember that this class does not exist. $this->missingClasses[$class] = true; } return $file; } private function findFileWithExtension($class, $ext) { // PSR-4 lookup $logicalPathPsr4 = strtr($class, '\\', DIRECTORY_SEPARATOR) . $ext; $first = $class[0]; if (isset($this->prefixLengthsPsr4[$first])) { $subPath = $class; while (false !== $lastPos = strrpos($subPath, '\\')) { $subPath = substr($subPath, 0, $lastPos); $search = $subPath.'\\'; if (isset($this->prefixDirsPsr4[$search])) { $pathEnd = DIRECTORY_SEPARATOR . substr($logicalPathPsr4, $lastPos + 1); foreach ($this->prefixDirsPsr4[$search] as $dir) { if (file_exists($file = $dir . $pathEnd)) { return $file; } } } } } // PSR-4 fallback dirs foreach ($this->fallbackDirsPsr4 as $dir) { if (file_exists($file = $dir . DIRECTORY_SEPARATOR . $logicalPathPsr4)) { return $file; } } // PSR-0 lookup if (false !== $pos = strrpos($class, '\\')) { // namespaced class name $logicalPathPsr0 = substr($logicalPathPsr4, 0, $pos + 1) . strtr(substr($logicalPathPsr4, $pos + 1), '_', DIRECTORY_SEPARATOR); } else { // PEAR-like class name $logicalPathPsr0 = strtr($class, '_', DIRECTORY_SEPARATOR) . $ext; } if (isset($this->prefixesPsr0[$first])) { foreach ($this->prefixesPsr0[$first] as $prefix => $dirs) { if (0 === strpos($class, $prefix)) { foreach ($dirs as $dir) { if (file_exists($file = $dir . DIRECTORY_SEPARATOR . $logicalPathPsr0)) { return $file; } } } } } // PSR-0 fallback dirs foreach ($this->fallbackDirsPsr0 as $dir) { if (file_exists($file = $dir . DIRECTORY_SEPARATOR . $logicalPathPsr0)) { return $file; } } // PSR-0 include paths. if ($this->useIncludePath && $file = stream_resolve_include_path($logicalPathPsr0)) { return $file; } return false; } } /** * Scope isolated include. * * Prevents access to $this/self from included files. */ function includeFile($file) { include $file; }

Leave a Reply

Your email address will not be published. Required fields are marked *